Probing DNA triple helix structure by chemical ligation

FEBS Letters

Shabarova, Zoe A.¹  Pyatrauskene, Olga V.¹  Dolinnaya, Nina G.¹ 

[1] A.N. Belozerski Laboratory and Chemical Department, Moscow State University, Moscow 119899, USSR

关键词: DNA triplex;    Chemical ligation or nucleic acids;    CD1;    1-ethyl-3-(3′-dimethyl-aminopropyl carbodiimide hydrochloride;    MES;    2-morpholinoethanesulfonate;   

DOI  :  10.1016/0014-5793(91)80692-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A series of oligomeric double and triple helical DNAs with irregular sequences of homopurine and homopyrimidine strands were prepared. DNA triplexes were identified by CD spectroscopy and thermal denaturation profiles (biphasic helix-coil transition). Condensation of oligonucleotides on single and double-stranded DNA templates was performed using water-soluble carbodiimide, phosphodiester and pyrophosphate internucleotide bonds being newly formed. Such chemical ligation proved to be a sensitive monitor of changes in the sugar-phosphate backbone resulting from conversion of double to triple helix and of third-strand binding.

【 授权许可】

Unknown