Modulation of α‐tropomyosin expression by α‐tocopherol in rat vascular smooth muscle cells

FEBS Letters

Aratri, Elisabetta¹  Breyer, Isabel¹  Azzi, Angelo¹  Spycher, Stefan E.¹ 

[1] Institut für Biochemie und Molekularbiologie, Universität Bern, Bühlstrasse 28, 3012 Bern, Switzerland

关键词: α-Tropomyosin;    α-Tocopherol;    β-Tocopherol;    Vitamin E;    Differential display;    Smooth muscle cell;   

DOI  :  10.1016/S0014-5793(99)00277-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of α-tocopherol (vitamin E) on gene expression in rat vascular smooth muscle cells was studied by the differential display technique. One gene out of about 1000 genes analyzed, identified as α-tropomyosin, showed an increased transcription level caused by α-tocopherol treatment. Northern and Western blot analysis revealed a time-dependent transient up-regulation of the amount of mRNA (peak between 2 and 3 h) and protein (peak at 5 h) in α-tocopherol-treated cells. No effect was observed in cells treated with β-tocopherol.

【 授权许可】

Unknown