Murine 12(R)‐lipoxygenase: functional expression, genomic structure and chromosomal localization 1

FEBS Letters

Marks, Friedrich¹  Bettenhausen, Rainer¹  Kinzig, Andreas¹  Fürstenberger, Gerhard¹  Krieg, Peter¹  Siebert, Malte¹ 

[1] Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany

关键词: Lipoxygenase;    Functional expression;    Gene structure;    Chromosomal localization;   

DOI  :  10.1016/S0014-5793(99)00196-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA, recently cloned (by Krieg et al. (1998)) from mouse skin, was shown to encode a 12(R)-lipoxygenase. When expressed in HEK cells, the recombinant protein converted methyl arachidonate into the corresponding 12-HETE ester which was shown to be the R-enantiomer by chiral phase chromatography. Neither arachidonic acid nor linoleic acid were substrates for the recombinant protein. The structure of the 12(R)-lipoxygenase gene is unique among all animal lipoxygenases in that it is divided into 15 exons and 14 introns spanning approximately 12.5 kb. By interspecific backcross analysis, the 12(R)-lipoxygenase gene was localized to the central region of mouse chromosome 11.

【 授权许可】

Unknown