Cloning of a novel four repeat protein related to voltage‐gated sodium and calcium channels

FEBS Letters

Cribbs, Leanne L.¹  Perez-Reyes, Edward¹  Lee, Jung-Ha¹ 

[1] Department of Physiology, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA

关键词: Amino acid sequence;    Calcium channel;    Sodium channel;    Gene library;    Molecular sequence data;    Protein conformation;    Structure-activity relationship;   

DOI  :  10.1016/S0014-5793(99)00082-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cloning has led to the discovery of more ion channels than predicted by functional studies, yet there remain channels that have not been cloned. We report the cloning of a novel protein that contains the four domain structure found in voltage-gated Ca²⁺ and Na⁺ channels. Phylogenetic relationships suggested that the protein might have diverged from an ancestral four repeat channel before the divergence of Ca²⁺ and Na⁺ channels. Northern blot analysis showed that mRNA transcripts encoding the protein are expressed predominantly in the brain, moderately in the heart, and weakly in the pancreas. Despite extensive expression attempts, currents from the putative channel were not detected. Based on its sequence, we propose that the novel protein might be a voltage-activated cation channel with unique gating properties.

【 授权许可】

Unknown