Genes for elongation factor EF‐1α in the brine shrimp Artemia

FEBS Letters

Lenstra, Johannes A.¹  Möller, Wim¹  van Hemert, Formijn J.¹ 

[1] Dept. Medical Biochemistry, Sylvius Laboratoria, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands

关键词: Artemia;    Elongation factor EF-1α;    Northern blot;    Southern blot;    Gene library;   

DOI  :  10.1016/0014-5793(83)80564-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A plasmid carrying a cDNA sequence coding for elongation factor EF-1α from Artemia was used to probe blots of mRNA and chromosomal DNA from Artemia. A messenger length for EF-1α corresponding to 1850 nucleotides was found. Southern blots pointed to a limited number (1–4) genes, coding for EF-1α. From an Artemia gene library a recombinant phage was isolated, which contains genomic sequences of EF-1α. S1-nuclease mapping indicated the presence of intervening sequences within this cloned gene.

【 授权许可】

Unknown