Rhophilin, a small GTPase Rho‐binding protein, is abundantly expressed in the mouse testis and localized in the principal piece of the sperm tail

FEBS Letters

Watanabe, Go³  Murata, Takahiko³  Nakamura, Ken-ichi³  Ishizaki, Toshimasa³  Mori, Chisato²  Watanabe, Naoki³  Fujita, Akiko³  Fujita, Jun¹  Narumiya, Shuh³  Yoshida, Osamu⁴ 

[1] Department of Clinical Molecular Biology, Kyoto University Faculty of Medicine, Kyoto 606, Japan;Department of Anatomy, Kyoto University Faculty of Medicine, Kyoto 606, Japan;Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606, Japan;Department of Urology, Kyoto University Faculty of Medicine, Kyoto 606, Japan

关键词: Rho;    Rhophilin;    Testis;    Sperm;    Sperm tail;    Fibrous sheath;    FITC;    fluorescein isothiocyanate;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/S0014-5793(99)00087-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tissue distribution and cellular localization of rhophilin, a 71 kDa Rho-binding protein, were examined in mice. Rhophilin mRNA was highly expressed in adult testis, but was absent in the testis of W/W^V mice deficient in germ cells. An anti-rhophilin antibody detected a band of an expected size in sperm extracts, which was enriched in the tail fraction. Immunofluorescence analysis revealed two lines of striated staining running in parallel in the principal piece of the sperm tail. These results suggest that rhophilin is expressed in germ cells and localized in the fibrous sheath of the sperm tail.

【 授权许可】

Unknown