Cytosolic phospholipase A2 in rat decidual cells: evidence for its role in decidualization

FEBS Letters

Hashimoto, I.¹  Kurusu, S.¹  Madarame, H.²  Endo, M.¹  Kawaminami, M.¹ 

[1] Laboratories of Veterinary Physiology, Kitasato University School of Veterinary Medicine and Animal Sciences, Towada, Aomori 034-8628, Japan;Experimental Animal Science, Kitasato University School of Veterinary Medicine and Animal Sciences, Towada, Aomori 034-8628, Japan

关键词: Cytosolic phospholipase A2;    Immunohistochemistry;    Decidualization;    Rat;    cPLA2;    cytosolic phospholipase A2;    PG;    prostaglandin;    AA;    arachidonic acid;    COX;    cyclooxygenase;    PAF;    platelet-activating factor;   

DOI  :  10.1016/S0014-5793(99)00078-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the existence and possible role of cytosolic phospholipase A₂ (cPLA₂) in rat decidualized uteri. PLA₂ activity in the cytosol of a decidualized uterine horn, induced by intraluminal oil infusion, was significantly higher than that in contralateral intact horn. The activity was almost completely depressed by cPLA₂ inhibitors including arachidonyl trifluoromethyl ketone (ATK). The immunoreactive signals for cPLA₂ were intense in decidua and glandular epithelial cells. In vivo administration of ATK (0.1–100 μg) caused a dose-dependent inhibition of decidualization. These results show the presence of cPLA₂ and its probable implication in decidualization in rat uterus.

【 授权许可】

Unknown