Hybridization of antisense oligonucleotides with the 3′ part of tRNAPhe

FEBS Letters

Petyuk, Vladislav A.²  Vlassov, Valentin V.²  Zenkova, Marina A.²  Giege, Richard¹ 

[1] Institut de Biologie Moléculaire et Cellulaire du CNRS, 15, rue R. Descartes, 67084 Strasbourg, France;Institute of Bioorganic Chemistry, Russian Academy of Sciences, 8, Lavrentiev Ave, Novosibirsk 630090, Russia

关键词: RNA structure;    Unfolding;    Complementary oligonucleotide;    Strand displacement;   

DOI  :  10.1016/S0014-5793(99)00063-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of antisense oligodeoxyribonucleotides with yeast tRNA^Phe was investigated. 14–15-mers complementary to the 3′-terminal sequence including the ACCA end bind to the tRNA under physiological conditions. At low oligonucleotide concentrations the binding occurs at the unique complementary site. At higher oligonucleotide concentrations, the second oligonucleotide molecule binds to the complex due to non-perfect duplex formation in the T-loop stabilized by stacking between the two bound oligonucleotides. In these complexes the acceptor stem is open and the 5′-terminal sequence of the tRNA is accessible for binding of a complementary oligonucleotide. The results prove that the efficient binding of oligonucleotides to the 3′-terminal sequence of the tRNA occurs through initial binding to the single-stranded sequence ACCA followed by invasion in the acceptor stem and strand displacement.

【 授权许可】

Unknown