Differential regulation of uncoupling protein‐1, ‐2 and ‐3 gene expression by sympathetic innervation in brown adipose tissue of thermoneutral or cold‐exposed rats

FEBS Letters

Moulin, Colette¹  Cohen-Adad, Frédérique¹  Lachuer, Joël¹  Duchamp, Claude¹  Barré, Hervé¹  Denjean, Frédérique¹  Géloën, Alain¹ 

[1] Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS-Université Cl. Bernard Lyon 1, Bât. 404, 4ème étage, 43 Bld du 11 Novembre 1918, F-69622 Villeurbanne Cedex, France

关键词: Unilateral sympathetic denervation;    Semi-quantitative reverse transcriptase polymerase chain reaction;    Cold exposure;   

DOI  :  10.1016/S0014-5793(99)00056-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The control of uncoupling protein-1, -2 and -3 (UCP-1, UCP-2, UCP-3) mRNA levels by sympathetic innervation in rats was investigated by specific and sensitive RT-PCR assays. In rats reared at thermoneutrality (25°C), unilateral surgical sympathetic denervation of interscapular brown adipose tissue (BAT) markedly reduced the UCP-1 mRNA level (−38%) as compared with the contralateral innervated BAT pad, but was without significant effect on UCP-2 and -3 mRNA levels. Cold exposure (7 days, 4°C) markedly increased UCP-1 (+180%), UCP-2 (+115%) and UCP-3 (+195%) mRNA levels in interscapular BAT. Unilateral sympathetic denervation prevented the cold-induced rise in BAT UCP-1 and UCP-2 mRNAs, but not that in BAT UCP-3 mRNA. Results were confirmed by Northern blot analysis. These data indicate a differential endocrine control of UCP-1, UCP-2 and UCP-3 gene expression in rat BAT both at thermoneutrality and during prolonged cold exposure.

【 授权许可】

Unknown