Transcription factor AP‐2 activity is modulated by protein kinase A‐mediated phosphorylation

FEBS Letters

Vázquez, Jesús¹  Marina, Anabel¹  Campillos, Mónica¹  Garcı́a, Miguel Angel¹  Valdivieso, Fernando¹ 

[1] Centro de Biologı́a Molecular Severo Ochoa, CSIC-Universidad Autónoma de Madrid, 28049 Madrid, Spain

关键词: AP-2;    cAMP;    Protein kinase A;    APOE promoter;    Alzheimer's disease;   

DOI  :  10.1016/S0014-5793(99)00021-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently reported that APOE promoter activity is stimulated by cAMP, this effect being mediated by factor AP-2 [Garcı́a et al. (1996) J. Neurosci. 16, 7550–7556]. Here, we study whether cAMP-induced phosphorylation modulates the activity of AP-2. Recombinant AP-2 was phosphorylated in vitro by protein kinase A (PKA) at Ser²³⁹. Mutation of Ser²³⁹ to Ala abolished in vitro phosphorylation of AP-2 by PKA, but not the DNA binding activity of AP-2. Cotransfection studies showed that PKA stimulated the effect of AP-2 on the APOE promoter, but not that of the S239A mutant. Therefore, cAMP may modulate AP-2 activity by PKA-induced phosphorylation of this factor.

【 授权许可】

Unknown