Fluorimetric multiparameter cell assay at the single cell level fabricated by optical tweezers

FEBS Letters

Seeger, Stefan¹  Zahn, Martin¹  Renken, Joachim¹ 

[1] Institut für Analytische Chemie, Chemo- und Biosensorik, University of Regensburg, D-93040 Regensburg, Germany

关键词: Optical tweezers;    Cellular screening;    Single cell technique;    Cell sensor;    Intracellular calcium;   

DOI  :  10.1016/S0014-5793(98)01693-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A fluorimetric multi-parameter cell sensor at the single cell level is presented which makes it possible to observe the physiological behavior of different cell lines, different physiological parameters, and statistical data at the same time. Different cell types were immobilized at predefined positions with high accuracy using optical tweezers and adhesion promoting surface layers. The process is applicable to both adherent and non-adherent cells. Coating of the immobilization area with mussel adhesive protein was shown to be essential for the process. Intracellular proton and calcium concentrations in different cell classes were simultaneously imaged and the specific activation of T lymphocytes was demonstrated. This method should be especially useful for drug screening due to the small sample volume and high information density.

【 授权许可】

Unknown