Mechanical manipulation of bone and cartilage cells with ‘optical tweezers’

FEBS Letters

Öberg, Åke²  Sundqvist, Tommy²  Walker, Lesley M.¹  El Haj, Alicia J.¹  Maxwell, Lori¹  Cooling, Leanne¹  Holm, Åsa² 

[1] Centre for Science and Technology in Medicine, School of Postgraduate Medicine, Keele University, North Staffs Hospital, Thornburrow Drive, Stoke on Trent ST4 7QB, UK;Division of Medical Microbiology, Department of Biomedical Engineering, Faculty of Health Sciences, Linkoping University, S-581 85 Linkoping, Sweden

关键词: Bone;    Cartilage;    Calcium;    Optical tweezers;    Force;   

DOI  :  10.1016/S0014-5793(99)01169-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The single beam optical gradient trap (optical tweezers) uses a single beam of laser light to non-invasively manipulate microscopic particles. Optical tweezers exerting a force of approximately 7 pN were applied to single bone and cartilage derived cells in culture and changes in intracellular calcium levels were observed using Fluo-3 labelling. Human derived osteoblasts responded to optical tweezers with an immediate increase in [Ca²⁺]_i that was inhibited by the addition of a calcium channel blocker nifedipine. Force applied to different regions of cells resulted in a variable response. [Ca²⁺]_i elevation in response to load was lower in rat femur derived osteoblasts, and not apparent in primary chondrocytes and the osteocytic cell line (MLO Y4).

【 授权许可】

Unknown