Alfalfa contains substantial 9‐hydroperoxide lyase activity and a 3Z:2E‐enal isomerase

FEBS Letters

Vliegenthart, Johannes F.G¹  Veldink, Gerrit A¹  Noordermeer, Minke A¹ 

[1] Bijvoet Center for Biomolecular Research, Department of Bio-Organic Chemistry, Utrecht University, Padualaan 8, NL-3584 CH Utrecht, The Netherlands

关键词: Fatty acid hydroperoxide lyase;    3Z:2E-enal isomerase;    Substrate specificity;    Medicago sativa L;    HPO;    hydroperoxide;    13-HPOD;    (9Z;    11E;    13S)-13-hydroperoxy-9;    11-octadecadienoic acid;    13-HPOT;    (9Z;    11E;    13S;    15Z)-13-hydroperoxy-9;    11;    15-octadecatrienoic acid;    9-HPOD;    (9S;    10E;    12Z)-9-hydroperoxy-10;    12-octadecadienoic acid;    9-HPOT;    (9S;    10E;    12Z;    15Z)-9-hydroperoxy-10;    12;    15-octadecatrienoic acid;    SPME;    solid phase micro-extraction;    DNPH;    2;    4-dinitrophenylhydrazine;   

DOI  :  10.1016/S0014-5793(98)01706-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fatty acid hydroperoxides formed by lipoxygenase can be cleaved by hydroperoxide lyase resulting in the formation of short-chain aldehydes and ω-oxo acids. Plant hydroperoxide lyases use 13- or 9-hydroperoxy linoleic and linolenic acid as substrates. Alfalfa (Medicago sativa L.) has been reported to contain a hydroperoxide lyase specific for 13-hydroperoxy linoleic and linolenic acid only. However, in addition to 13-hydroperoxide lyase activity we found substantial 9-hydroperoxide lyase activity in alfalfa seedlings as well. The specific activity for 9-hydroperoxy fatty acids was about 50% of the activity for the 13-isomers. Furthermore, alfalfa seedlings contain a 3Z:2E-enal isomerase that converts the 3Z-enal products to their 2E-enal isoforms.

【 授权许可】

Unknown