HIV‐1 gp160 decreases the K+ voltage‐gated current from Jurkat E6.1 T cells by up‐phosphorylation

FEBS Letters

Rona, Jean-Pierre¹  Dellis, Olivier¹  Bouteau, François¹  Guenounou, Moncef² 

[1] Laboratoire d'Electrophysiologie des Membranes, LPCMSP, Université Denis Diderot, Paris 7, 2 place Jussieu, F-75251 Paris Cedex 05, France;Laboratoire d'Immunologie et Virologie Appliquées, Faculté de Pharmacie, Université de Reims, 51 rue Cognacq-Jay, F-51110 Reims Cedex 01, France

关键词: Human T cell;    Potassium channel;    HIV-1 gp160;    Protein kinase C;    Membrane potential;    p56lck;    HIV-1;    human immunodeficiency virus type 1;    PKC;    protein kinase C;    OA;    okadaic acid;    Stauro;    staurosporine;    TPA;    12-O-tetradecanoyl phorbol 13-acetate;    E m;    membrane potential;    4-AP;    4-aminopyridine;   

DOI  :  10.1016/S0014-5793(98)01691-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

HIV-1 gp120/gp160 is known to disturb the activity of p56^lck, protein kinase C (PKC) and Ca²⁺ homeostasis in T lymphocytes. We found that gp160 decreases the Kv1.3 current of Jurkat E6.1 cells probably by increasing the PKC-dependent phosphorylation of Kv channel protein after 5 days. This decrease is dose-dependent. In contrast, gp160 did not decrease the Kv1.3 current of the JCaM1.6 cell line, a p56^lck-defective Jurkat cell line. This shows that p56^lck was at the beginning of the events which induced the Kv1.3 current decrease. As a consequence of this decrease, Jurkat E6.1 cells were depolarized and exhibited a volume increase.

【 授权许可】

Unknown