期刊论文详细信息
FEBS Letters
AT2 receptor stimulation induces generation of ceramides in PC12W cells
Busche, Silke1  Gallinat, Stefan1  Krönke, Martin2  Schütze, Stefan2  Unger, Thomas1 
[1] Institute of Pharmacology, Christian-Albrechts-University of Kiel, Hospitalstr. 4, 24105 Kiel, Germany;Institute of Immunology, German Institute for High Blood Pressure Research, 69120 Heidelberg, Germany
关键词: Angiotensin II;    AT2 receptor;    Ceramide;    Apoptosis;    PC12W cell;   
DOI  :  10.1016/S0014-5793(98)01675-5
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

The angiotensin AT2 receptor has been implicated in both regeneration and apoptosis. To further investigate the molecular mechanisms leading to AT2 receptor-induced programmed cell death in PC12W cells we studied the effects of angiotensin II (ANG II) on ceramide levels by HPTLC analysis. We could demonstrate that ANG II time- (1–10 h) and dose-dependently (10−8–5×10−6 M) increased ceramide levels by maximally 175% but did not affect sphingomyelin degradation. The ANG II effects were mediated by AT2 receptors since they were completely abolished by co-incubation with the AT2 receptor antagonist, PD123177 (10−5 M), but not by the AT1 receptor antagonist, losartan (10−5 M). These data suggest a novel signal transduction pathway to the AT2 receptor leading to apoptosis in neuronal cells.

【 授权许可】

Unknown   

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