Dexamethasone modulation of multidrug transporters in normal tissues

FEBS Letters

Demeule, Michel¹  Brossard, Mathieu¹  Beaulieu, Édith¹  Béliveau, Richard¹  Jodoin, Julie¹ 

[1] Laboratoire d'Oncologie Moléculaire et Centre de Cancérologie Charles Bruneau, Université du Québec à Montréal, C.P. 8888, Succ. Centre-ville, Montréal, Que. H3C 3P8, Canada

关键词: Dexamethasone;    P-glycoprotein;    cMOAT;    Mrp2;    Protein expression;    BBM;    brush border membranes;    ECL;    enhanced chemiluminescence;    IAAP;    [125I]iodoaryl azidoprazosin;    mAb;    monoclonal antibody;    pAb;    polyclonal antibody;    P-gp;    P-glycoprotein;    PVDF;    polyvinylidene difluoride;    spgp;    sister P-glycoprotein;    TBS-T;    Tris buffered saline with 0.3% Tween 20;   

DOI  :  10.1016/S0014-5793(98)01663-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of P-glycoprotein (P-gp) and canalicular multispecific organic anion transporter (cMOAT or Mrp2) was evaluated by Western blotting analysis of rat tissues isolated following daily administration (1 mg kg⁻¹ day⁻¹) of dexamethasone over 4 days. Dexamethasone rapidly increased P-gp expression more than 4.5- and 2-fold in liver and lung, respectively, while it was decreased 40% in kidney. cMOAT expression was increased 2-fold in liver and kidney following dexamethasone treatment. The levels of both proteins returned to control values by 6 days after the conclusion of dexamethasone administration. These results indicate that dexamethasone can modulate P-gp and cMOAT expression in specific rat tissues and may have significant relevance for patients treated with dexamethasone as a single agent or in combination therapy with other drugs.

【 授权许可】

Unknown