Interferon‐γ inhibits the myofibroblastic phenotype of rat palatal fibroblasts induced by transforming growth factor‐β1 in vitro

FEBS Letters

Kuroda, Takayuki³  Baba, Yoshiyuki³  Yokozeki, Masahiko³  Moriyama, Keiji¹  Shimokawa, Hitoyata² 

[1] Department of Orthodontics, School of Dentistry, University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan;Department of Biochemistry, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima, 1-chome, Bunkyo-ku, Tokyo 113-8549, Japan;Second Department of Orthodontics, Faculty of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima, 1-chome, Bunkyo-ku, Tokyo 113-8549, Japan

关键词: Transforming growth factor-β;    Interferon-γ;    Myofibroblast;    Scar;    Wound healing;    α-Smooth muscle actin;   

DOI  :  10.1016/S0014-5793(98)01626-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interferon-γ (IFN-γ), a multifunctional cytokine, has been noted as a potential therapeutic agent for various fibrotic disorders, including excessive scar tissue formation. We previously reported that transforming growth factor-β1 (TGF-β1) induced the myofibroblastic phenotype in palatal fibroblasts derived from palatal mucosa, and that such effects might have a close link to palatal scar formation. In the present study, we examined the effects of IFN-γ on TGF-β1-pretreated palatal fibroblasts for the purpose of clarifying the suppressive potency against myofibroblastic phenotype expression in vitro. IFN-γ significantly altered the spindle morphology of TGF-β1-pretreated palatal fibroblasts into the polygonal one that was similar to the non-treated palatal fibroblasts. This change was parallel with a decrease in the expression of α-smooth muscle actin protein, a marker for myofibroblast, as determined by immunoblot analysis. Northern blot analysis showed that IFN-γ inhibited proα2(I) collagen mRNA expression that was stimulated by TGF-β1 pretreatment for 24 h. Furthermore, IFN-γ decreased the cell contractility enhanced by TGF-β1 pretreatment for 24 h in a three-dimensional collagen gel culture system. These results suggest that IFN-γ may have negative effects with regard to controlling the myofibroblastic phenotype induced by TGF-β1 in palatal fibroblasts.

【 授权许可】

Unknown