Regulation of voltage‐dependent K+ channels by methionine oxidation: effect of nitric oxide and vitamin C

FEBS Letters

Weissbach, Herbert²  Hoshi, Toshinori¹  Heinemann, Stefan H.³  Ciorba, Matthew A.¹  Brot, Nathan⁴ 

[1] Department of Physiology and Biophysics, The University of Iowa, Iowa City, IA 52242, USA;Department of Biological Sciences, Florida Atlantic University, Boca Raton, FL 33431, USA;Max-Planck-Gesellschaft, Research Unit Molecular and Cellular Biophysics, Drackendorfer Str. 1, D-07747 Jena, Germany;Hospital for Special Surgery, Cornell University Medical Center, New York, NY 10021, USA

关键词: Methionine oxidation;    Nitric oxide;    Antioxidant;    A-type potassium channel;    Xenopus oocyte;   

DOI  :  10.1016/S0014-5793(98)01616-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methionine oxidation is known to alter functional properties of a transient A-type potassium channel expressed in Xenopus oocytes. We show here that nitric oxide (NO) slows down the K⁺ channel inactivation time course by oxidizing a critical methionine residue in the inactivation ball domain of the channel protein. We also demonstrate that the channel protein is protected from methionine oxidation by the enzyme methionine sulfoxide reductase and the antioxidant vitamin C.

【 授权许可】

Unknown