Functional reconstitution of RNase P activity from a plastid RNA subunit and a cyanobacterial protein subunit

FEBS Letters

Vioque, Agustı́n¹  Pascual, Alberto¹ 

[1] Instituto de Bioquı́mica Vegetal y Fotosı́ntesis, Centro de Investigaciones Cientı́ficas Isla de la Cartuja, Universidad de Sevilla-CSIC, Avenida Americo Vespucio s/n, 41092 Sevilla, Spain

关键词: Plastid evolution;    RNA catalysis;    Ribonuclease P;    Cyanobacteria;    Cyanophora cyanelle;    P RNA;    RNA subunit of RNase P;    P protein;    protein subunit of RNase P;    pre-tRNA;    precursor of tRNA;   

DOI  :  10.1016/S0014-5793(98)01621-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The plastid (cyanelle) from the Glaucocystophyceae alga Cyanophora paradoxa contains an RNase P RNA subunit (P RNA) similar to the cyanobacterial P RNA. We have synthesized this RNA by in vitro transcription and analyzed its activity in the absence or presence of the RNase P protein subunit (P protein) from Escherichia coli and the cyanobacterium Synechocystis sp. PCC 6803. In contrast to the bacterial P RNA, the cyanelle P RNA is not active in the absence of protein in any of the conditions tested. A functional enzyme could be reconstituted with the Synechocystis protein but not with the E. coli protein. This is the first demonstration of RNase P activity reconstitution from organellar and bacterial subunits.

【 授权许可】

Unknown