In vitro import of pre‐ferredoxin‐NADP+‐oxidoreductase from Cyanophora paradoxa into cyanelles and into pea chloroplasts

FEBS Letters

Bohnert, Hans J.¹  Schenk, Hainfried E.A.³  Jakowitsch, Johannes²  Löffelhardt, Wolfgang²  Steiner, Jürgen²  Ma, Yan²  Neumann-Spallart, Christoph² 

[1] Department of Biochemistry, University of Arizona, Tucson, AZ 85721, USA;Institut für Biochemie und Molekulare Zellbiologie der Universität Wien und Ludwig-Boltzmann-Forschungsstelle fü Biochemie, Dr. Bohrgasse 9, A-1090 Vienna, Austria;Botanisches Institut der Universität Tübingen, D-72076 Tübingen, Germany

关键词: Pre-ferredoxin-NADP+-oxidoreductase;    Import;    in vitro;    Cyanelles;    Cyanophora paradoxa;    Pea chloroplast;    Plastid evolution;   

DOI  :  10.1016/0014-5793(96)00102-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a novel cyanelle isolation procedure we showed that pre-ferredoxin-NADP⁺-oxidoreductase (pre-FNR) from C. paradoxa is translocated in vitro across the peptidoglycancontaining cyanelle envelope. Efficient import was also observed in a heterologous system with pea chloroplasts as the recipient organelles. These results support the conclusion derived from comparative analysis of plastid genome organization, that all plastids originate from a common semi-autonomous endosymbiotic ancestor.

【 授权许可】

Unknown