Studies on the import into mitochondria of yeast ATP synthase subunits 8 and 9 encoded by artificial nuclear genes

FEBS Letters

Devenish, Rodney J.¹  Law, Ruby H.P.¹  Nagley, Phillip¹  Nero, Debra¹  Farrell, Leigh B.¹ 

[1] Department of Biochemistry and Centre for Molecular Biology and Medicine, Monash University, Clayton, Victoria 3168, Australia

关键词: Import;    Mitochondrial ATPase complex;    Membrane assembly;    Gene relocation;    Site-directed mutagenesis;    (Saccharomyces cerevisiae);    mtATPase;    mitochondrial proton-translocating ATPase (ATP synthase);    N9L;    cleavable N-terminal leader from the presequence of Neurospora crassa mtATPase subunit 9;    Y8 and Y9;    subunits 8 and 9;    respectively;    of yeast mtATPase;   

DOI  :  10.1016/0014-5793(88)80086-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Direct fusions have been constructed between each of subunits 8 and 9 from mitochondrial ATPase of Saccharomyces cerevisiae, proteins normally encoded inside mitochondria, and the cleavable N-terminal transit peptide from the nuclearly encoded precursor to subunit 9 of Neurospora crassa mitochondrial ATPase. The subunit 8 construct was imported efficiently into isolated yeast mitochondria and was processed at or very near the fusion point. When expressed in vivo from its artificial nuclear gene, this cytoplasmically synthesized form of subunit 8 restored the growth defects of aap1 mutants unable to produce subunit 8 inside the mitochondria. The subunit 9 construct was, however, unable to be imported into isolated mitochondria and could not, following nuclear expression in vivo, complement growth defects in mitochondrial oli1 mutants. This behaviour is contrasted with the previously demonstrated import competence of another yeast subunit 9 fusion, bearing the first five residues of mature N. crassa subunit 9 interposed between its own transit peptide and the yeast subunit 9 moiety.

【 授权许可】

Unknown