Localization of Rho GTPase in sea urchin eggs

FEBS Letters

Mabuchi, Issei¹  Nishimura, Yukako¹  Nakano, Kentaro¹ 

[1] Division of Biology, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan

关键词: Sea urchin egg;    Cytokinesis;    Rho GTPase;    Cleavage furrow;    Contractile ring;    Midbody;    DAPI;    4′;    6-diamidino-2-phenylindole;    Bodipy;    4;    4-difluoro-4-bora-3a;    4a-diaza-s-indacene;   

DOI  :  10.1016/S0014-5793(98)01531-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated the urho1 (rchin in English or ni in Japanese) gene from the sea urchin cDNA library which encodes a Rho GTPase. Anti-URho1 antibodies specifically recognized a 22 kDa protein in the extracts of echinoderm eggs. URho1 was concentrated in the cortices from both unfertilized and fertilized eggs as judged by immunoblot analysis. URho1 may bind directly to the cell membrane but not be a component of the cortical layer. Immunofluorescence microscopy revealed that URho1 is localized to the cleavage furrow and the midbody during cytokinesis.

【 授权许可】

Unknown