Specific detections of the early process of the glycation reaction by fructose and glucose in diabetic rat lens

FEBS Letters

Fujii, Junichi²  Hoshi, Ayumu²  Miyazawa, Nobuko²  Tano, Yasuo¹  Okado, Ayako²  Taniguchi, Naoyuki²  Kawasaki, Yoshimi² 

[1] Department of Ophthalmology, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan;Department of Biochemistry, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan

关键词: Glycation;    Polyol pathway;    Fructation;    Glucation;    Crystallin;    Amadori product;    AGE;    advanced glycation end products;    ELISA;    enzyme linked immunosorbent assay;    STZ;    streptozotocin;    AR;    aldose reductase;    SDH;    sorbitol dehydrogenase;    RP-HPLC;    reverse phased-high performance liquid chromatography;    PBS;    phosphate-buffered saline;    TBS;    Tris-buffered saline;    BSA;    bovine serum albumin;    α-CHCA;    α-cyanohydroxycinnamic acid;    MALDI-TOF-MS;    matrix-assisted laser desorption/ionization time-of-flight mass spectrometry;   

DOI  :  10.1016/S0014-5793(98)01529-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The glycation reaction by fructose, as well as that by glucose, in control and diabetic rat lens was analyzed by using antibodies which specifically recognize adducts of lysine with fructose and with glucose. Levels of fructose adducts in diabetic rat lens were 2.5 times that of the control, and correlated with sorbitol levels. This was mainly due to enhanced glycation of β- and γ-crystallins by fructose under diabetic conditions. These data suggest that glycation by fructose may also play a role in cataract formation under conditions of diabetes and aging.

【 授权许可】

Unknown