Methylglyoxal and the polyol pathway

FEBS Letters

McKinley-McKee, John S.¹  Lindstad, Rune I.¹ 

[1] Biochemical Institute, University of Oslo, P.O. Box 1041, N-0316 Oslo, Norway

关键词: Methylglyoxal;    Polyol pathway;    Sorbitol dehydrogenase;    Sheep liver;    Enzymes: sorbitol dehydrogenase;    l-iditol:NAD 2-oxidoreductase (EC 1.1.1.14);    aldose reductase;    alditol:NAD(P) 1-oxidoreductase(EC 1.1.1.21);    glyoxalase I (EC 4.4.1.5);    glyoxalase II (EC 3.1.2.6);   

DOI  :  10.1016/0014-5793(93)80913-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methylglyoxal, 1,2-propanediol and glycerol are shown to be substrates for sheep liver sorbitol dehydrogenase. With 1,2-propanediol the enzymecatalyzed reaction occurs specifically with the R(⁻)-enantiomer. The maximum velocities and the specificity constants obtained for the three-carbon substrates are considerably lower than those reported previously for sorbitol, and suggest that rate-determination is imposed by catalytic steps other than the enzyme-coenzyme product dissociation. The present findings are discussed in terms of substrate specificity and stereospecificity, and may indicate novel aspects of sorbitol dehydrogenase function in relation to glucose metabolism and diabetic pathogenesis.

【 授权许可】

Unknown