A protein import receptor in pea chloroplasts, Toc86, is only a proteolytic fragment of a larger polypeptide

FEBS Letters

Bölter, Bettina¹  May, Timo¹  Soll, Jürgen¹ 

[1] Botanisches Institut, Universität Kiel, Am Botanischen Garten 1–5, D-24118 Kiel, Germany

关键词: Chloroplast;    Protein import;    Precursor protein;    Receptor;    Pisum sativum;    preSSU;    precursor form of ribulose-1;    5-bisphosphate carboxylase;    mSSU;    mature form of preSSU;   

DOI  :  10.1016/S0014-5793(98)01525-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The protein import complex of the chloroplastic outer envelope (Toc-complex) contains a prominent subunit of 86 kDa molecular weight (Toc86). Toc86 was identified as a putative precursor receptor. The Arabidopsis genome sequencing project indicates that Toc86 represents only a proteolytic fragment of a larger polypeptide of 160 kDa. The 160-kDa protein, which we name Toc160, is only present in significant amounts in pea chloroplasts isolated under stringent conditions. The capacity of chloroplasts to import an in vitro translated precursor protein correlates well with the integrity of Toc160. We conclude that Toc160 is still a bonafide subunit of the protein import machinery of chloroplasts.

【 授权许可】

Unknown