Increased uncoupling protein‐2 and ‐3 gene expressions in skeletal muscle of STZ‐induced diabetic rats

FEBS Letters

Kageyama, Haruaki²  Osaka, Toshimasa²  Nemoto, Kiyomitsu⁴  Inoue, Shuji²  Oka, Jun²  Giacobino, Jean-Paul³  Kashiwa, Takayuki²  Kashiba, Misato²  Ricquier, Daniel⁵  Suga, Asako¹  Namba, Yoshio²  Hirano, Tsutomu¹ 

[1] First Department of Internal Medicine, Showa University School of Medicine, 1-5-8 Hatanodai Shinagawa-ku, Tokyo, 142-8666, Japan;Division of Geriatric Health and Nutrition, National Institute of Health and Nutrition, 1-23-1 Toyama Shinjuku-ku, Tokyo, 162-8636, Japan;Department of Medical Biochemistry, Faculty of Medicine, University of Geneva, 1211 Geneva 4, Switzerland;Laboratory of Health Science, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka-shi, 422-8526, Japan;Centre de Recherche sur l'Endocrinologie Moléculaire et le Développement, Centre National de la Recherche Scientifique, 9, rue Jules Hetzel, 92190 Meudon, France

关键词: Streptozotocin;    Diabetic rat;    Uncoupling protein;    Gastrocnemius muscle;    Brown adipose tissue;    Gene expression;   

DOI  :  10.1016/S0014-5793(98)01506-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Streptozotocin (STZ)-induced diabetic animals are vulnerable to cold stress. Uncoupling proteins (UCPs) play an important role in regulating thermogenesis. We investigated the gene expressions of UCPs in brown adipose tissue (BAT), white adipose tissue (WAT), liver and gastrocnemius muscle of STZ-diabetic rats using Northern blot. UCP-1, -2 and -3 mRNA expressions in BAT were all remarkably lower in STZ-diabetic rats than those in control rats. Both UCP-2 and -3 gene expressions in gastrocnemius muscle were substantially elevated in STZ-diabetic rats and insulin treatment restored UCP gene expressions to normal levels. These results suggest that in STZ-diabetic rats, the overexpression of UCP-2 and UCP-3 in skeletal muscle provides a defense against hypothermogenesis caused by decreased UCPs in BAT.

【 授权许可】

Unknown