The stereochemical course of the reaction mechanism of trehalose phosphorylase from Schizophyllum commune

FEBS Letters

Eis, Christian¹  Dax, Karl²  Albert, Martin²  Nidetzky, Bernd¹ 

[1] Division of Biochemical Engineering, Institute of Food Technology, Universität für Bodenkultur (BOKU), Muthgasse 18, A-1190 Wien, Austria;Institute of Organic Chemistry, Technical University of Graz, Stremayrgasse 18, A-8010 Graz, Austria

关键词: Trehalose phosphorylase;    Stereochemistry;    Retaining mechanism;    d-Glc 1-P;    d-glucose 1-phosphate;    α-d-Glc 1-F;    α-d-glucose 1-fluoride;   

DOI  :  10.1016/S0014-5793(98)01504-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorolysis of α,α-trehalose catalyzed by trehalose phosphorylase from the basidiomycete Schizophyllum commune proceeds via net retention of anomeric configuration and yields α-d-glucose 1-phosphate and α-d-glucose as the products. In reverse reaction, only the α-anomers of d-glucose 1-phosphate and d-glucose are utilized as glucosyl donor and acceptor, respectively, and give exclusively the α,α-product. Trehalose phosphorylase converts α-d-glucose 1-fluoride and phosphate into α-d-glucose 1-phosphate, a reaction requiring the stereospecific protonation of the glucosyl fluoride by a Brønsted acid. The results are discussed with regard to a plausible reaction mechanism of fungal trehalose phosphorylase.

【 授权许可】

Unknown