Inhibition of skeletal muscle sarcoplasmic reticulum Ca2+‐ATPase by nitric oxide

FEBS Letters

Saitoh, Noriko²  Nishio, Hideaki²  Hata, Fumiaki¹  Ishii, Toshiaki¹  Takeuchi, Tadayoshi¹  Sunami, Osamu² 

[1] Department of Molecular Physiology and Biochemistry, Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai 599-8531, Japan;Department of Veterinary Pharmacology, College of Agriculture, Osaka Prefecture University, Sakai 599-8531, Japan

关键词: Sarcoplasmic reticulum Ca2+-ATPase;    Nitric oxide;    Sarcoplasmic reticulum;    Ca2+ uptake;    Protein kinase G;    SERCA;    sarcoplasmic reticulum Ca2+-ATPase;    SR;    sarcoplasmic reticulum;    PKG;    cyclic GMP-dependent protein kinase;    DTT;    dithiothreitol;   

DOI  :  10.1016/S0014-5793(98)01460-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of nitric oxide on the activities of thapsigargin-sensitive sarcoplasmic reticulum Ca²⁺-ATPase (SERCA) and Ca²⁺ uptake by sarcoplasmic reticulum (SR) membranes prepared from white skeletal muscle of rabbit femoral muscle were studied. Pretreatment of the SR preparations with nitric oxide at concentrations of up to 250 μM for 1 min decreased the SERCA activity concentration dependently, and also decreased their Ca²⁺ uptake. Both these effects of nitric oxide were reversible. Inhibitors of guanylyl cyclase and protein kinase G (PKG) had no significant effect on the nitric oxide-induced inhibitions of SERCA and Ca²⁺ uptake. Moreover, dithiothreitol did not reverse the inhibitory effects of nitric oxide on SERCA and Ca²⁺ uptake. These findings suggest that nitric oxide inhibits SERCA, mainly SERCA 1, of rabbit femoral skeletal muscle by an action independent of the cyclic GMP-PKG system or oxidation of thiols, and probably by a direct action on SERCA protein.

【 授权许可】

Unknown