Separation of active and inactive (nonphosphorylating) Ca2+‐ATPase in sacroplasmic reticulum subfractions from low‐frequency‐stimulated rabbit muscle

FEBS Letters

Matsushita, Shigeki¹  Pette, Dirk¹  Dux, László¹ 

[1] Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750 Konstanz, Germany

关键词: Low-frequency stimulation;    Sarcoplasmic reticulum Ca2+-ATPase;   

DOI  :  10.1016/0014-5793(91)80669-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chronic low-frequency stimulation elicits in rabbit fast-twitch muscle a partial inactivation of the sarcoplasmic reticulum (SR) Ca²⁺-ATPase and Ca²⁺-uptake activities. Inactive Ca²⁺-ATPase was enriched in a light microsomal fraction by sucrose density gradient centrifugation after calcium oxalate loading in the presence of ATP. This fraction showed a reduced specific activity and phosphoprotein formation of the Ca²⁺-transport ATPase. These results suggest that the inactivation of the Ca²⁺-ATPase as induced by increased contractile activity, is confined to a specific SR vesicle population.

【 授权许可】

Unknown