An upstream negative regulatory element in human granulocyte‐macrophage colony‐stimulating factor promoter is recognised by AP1 family members

FEBS Letters

Bianchi-Scarrà, Giovanna¹  Garrè, Cecilia¹  Gramigni, Claudia¹  Penco, Silvana¹  Ravazzolo, Roberto¹ 

[1] Department of Clinical and Experimental Oncology, University of Genoa, Viale Benedetto XV, 6, 16132 Genoa, Italy

关键词: Granulocyte-macrophage colony-stimulating factor;    AP1;    U87MG;    Transcriptional regulation;    Negative cis element;    GM-CSF;    granulocyte-macrophage colony-stimulating factor;    TPA;    12-O-tetradecanoyl phorbol-13-acetate;    TRE;    TPA responsive element;    CAT;    chloramphenicol acetyltransferase;    RT-PCR;    reverse transcriptase polymerase chain reaction;    EMSA;    electrophoretic mobility shift assay;   

DOI  :  10.1016/S0014-5793(98)01443-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine involved in haematopoiesis and host defence. Production of GM-CSF has been detected in tumour cells including the U87MG astrocytoma cell line. Previous studies have been focused on the regulatory role of the proximal region of the GM-CSF promoter. Our studies on the distal region of the promoter in U87MG cells identify a negative cis element (−1377/−1298) which contains a AP1-like site able to bind c-jun and c-fos transcription factors, according to the results of DNA/protein binding assays. Mutagenesis of the AP1-like site eliminates AP1 binding and the negative effect on promoter activity.

【 授权许可】

Unknown