12‐Bromododecanoic acid binds inside the calyx of bovine β‐lactoglobulin

FEBS Letters

Baker, Edward N³  Jameson, Geoffrey B²  Qin, Bin Y²  Creamer, Lawrence K¹ 

[1] New Zealand Dairy Research Institute, Palmerston North, New Zealand;Centre for Structural Biology, Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand;Centre for Structural Biology, Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand

关键词: Bovine β-lactoglobulin;    Ligand binding;    Fatty-acid complex;    X-ray crystal structure;   

DOI  :  10.1016/S0014-5793(98)01199-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The X-ray structure of bovine β-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 Å in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carboxylate head group lies at the surface of the molecule, and the lid to the calyx is open at the pH of crystallization (pH 7.3), consistent with the conformation observed in ligand-free bovine β-lactoglobulin in lattice Z at pH 7.1 and pH 8.2.

【 授权许可】

Unknown