Ligand‐binding specificity of human fibroblast growth factor receptor‐3 IIIc

FEBS Letters

Kaplow, June²  Hayman, Michael J.³  Jaye, Michael²  Lin, Hsien-Yi¹ 

[1] Graduate Program in Molecular Biology and Biochemistry, State University of New York at Stony Brook, Stony Brook, NY 11794-5222, USA;Rhône-Poulenc Rorer Central Research, 500 Arcola Road NW14, Collegeville, PA 19426, USA;Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, NY 11794-5222, USA

关键词: Ligand binding;    Fibroblast growth factor;    Fibroblast growth factor receptor;   

DOI  :  10.1016/S0014-5793(97)00739-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Earlier studies indicated that human fibroblast growth factor receptor (FGFR)-3 IIIc was activated equally well by both FGF-1 and FGF-2. In contrast, murine FGFR-3 IIIc was preferentially activated by FGF-1. To address this issue, we determined the ligand-binding specificity of human FGFR-3 IIIc in comparison with human FGFR-1 IIIc. By equilibrium binding human FGFR-3 IIIc preferentially bound FGF-1 with high affinity, whereas FGFR-1 IIIc bound both FGF-1 and -2 with high affinity. By competition binding using FGF-1, -2, -4, or -6, FGF-1 competed more efficiently than the other FGFs. These results suggest that like the murine FGFR-3 III, FGF-1 is a preferred ligand for human FGFR-3 IIIc.

【 授权许可】

Unknown