The Pur10 protein encoded in the gene cluster for puromycin biosynthesis of Streptomyces alboniger is an NAD‐dependent ATP dehydrogenase

FEBS Letters

Jiménez, Antonio¹  Tercero, José Antonio¹  Espinosa, Juan Carlos¹  Rubio, Miguel Angel¹ 

[1] Centro de Biologı́a Molecular ‘Severo Ochoa’ (CSIC/UAM), Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain

关键词: pur cluster;    Puromycin biosynthesis;    Pur10;    ATP dehydrogenase;    Streptomyces;    aa;    amino acid(s);    LB;    Luria-Bertani broth;    nt;    nucleotide(s);   

DOI  :  10.1016/S0014-5793(98)01228-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The pur10 gene of the puromycin (pur) cluster of Streptomyces alboniger is essential for the biosynthesis of this antibiotic. Highly purified Pur10 protein, obtained in Escherichia coli as a recombinant protein fused to a histidine tail, had an NAD-dependent ATP dehydrogenase activity. The K _m and V _max values for ATP were 0.49 mM and 14.5 nmol/min and for NAD 0.53 mM and 15.2 nmol/min, respectively. The ATP-derived product of the reaction apparently decomposed producing a triphosphorylated compound plus an adenine derivative. These and previous results suggested that Pur10 carries out the first step of the puromycin biosynthetic pathway, namely, conversion of ATP into 3′-keto-3′-deoxyATP.

【 授权许可】

Unknown