Expression of the Streptomyces alboniger pur cluster in Streptomyces lividans is dependent on the bldA‐encoded tRNALeu

FEBS Letters

Jiménez, Antonio¹  Tercero, José Antonio¹  Espinosa, Juan Carlos¹ 

[1] Centro de Biologı́a Molecular ``Severo Ochoa' (C.S.I.C./U.A.M.), Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain

关键词: pur cluster;    Puromycin biosynthesis;    bldA gene;    pur regulation;    Streptomyces;   

DOI  :  10.1016/S0014-5793(97)01564-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Streptomyces lividans 1326-9, a bldA ⁺ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA ⁺ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so at drastically decreased levels. Transformation of low producers with the wild-type bldA gene reverted this phenotype to normal production. These data, in addition to the presence of a TTA codon in the amino-terminal coding region of the pur10 and pur6 genes of the pur cluster, suggest that the puromycin biosynthetic pathway is translationally dependent on the bldA gene product, a tRNA^Leu.

【 授权许可】

Unknown