Trp proteins form store‐operated cation channels in human vascular endothelial cells

FEBS Letters

Hingel, Susanne²  Zhu, Xi³  Groschner, Klaus²  Schreibmayer, Wolfgang¹  Balzer, Monika²  Lintschinger, Birgit²  Romanin, Christoph⁴ 

[1]Institut für Medizinische Physik und Biophysik, Karl-Franzens-Universität Graz, Graz, Austria

[2]Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Universitätsplatz 2, A-8010 Graz, Austria

[3]Department of Neurobiotechnology, Ohio State University, Columbus, OH, USA

[4]Institut für Biophysik, Johannes-Kepler-Universität Linz, Linz, Austria

关键词: Vascular endothelium;    Store-operated cation channel;    Trp channel;    Protein;    IP3;    BAPTA;    1;    2-bis(2-aminophenoxy)ethane-N;    N;    N′;    N′-tetraacetic acid;    ECV 304;    cell line from human umbilical vein endothelium;    GFP;    green fluorescent protein;    HUVEC;    human umbilical vein endothelial cells;    HEK 293;    cell line from human embryonic kidney cells;    HEPES;    N-2-hydroxyethylpiperazine-N′-ethanesulfonic acid;    N-TRP;    N-terminal domain (residues 1–302) of hTrp3;   

DOI  :  10.1016/S0014-5793(98)01212-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Members of the Trp protein family have been suggested as the structural basis of store-operated cation conductances. With this study, we provide evidence for the expression of three isoforms of Trp (hTrp1, 3 and 4) in human umbilical vein endothelial cells (HUVEC). The role of Trp proteins in store regulation of endothelial membrane conductances was tested by expression of an N-terminal fragment of hTrp3 (N-TRP) which exerts a dominant negative effect on Trp channel function presumably due to suppression of channel assembly. Depletion of intracellular Ca²⁺ stores with IP₃ (100 μM) or thapsigargin (100 nM) induced a substantial cation conductance in sham-transfected HUVEC as well as in HUVEC transfected with hTrp3. In contrast, HUVEC transfected with N-TRP failed to exhibit store-operated currents. Our results suggest the involvement of Trp related proteins in the store-operated cation conductance of human vascular endothelial cells.

【 授权许可】

Unknown