Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X‐ray crystallography

FEBS Letters

Goodenough, Peter W¹  Woo, Eui-Jeon¹  Dunwell, Jim M¹  Pickersgill, Richard W² 

[1] School of Plant Sciences, The University of Reading, Whiteknights P.O. Box 221, Reading RG6 6AS, UK;Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknights Road, Reading RG6 6BZ, UK

关键词: Germin;    Cupin;    Seed storage protein;    Abiotic stress;    Pathogenesis-related protein;    Hydrogen peroxide;   

DOI  :  10.1016/S0014-5793(98)01203-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The oxalate oxidase enzyme expressed in barley roots is a thermostable, protease-resistant enzyme that generates H₂O₂. It has great medical importance because of its use to assay plasma and urinary oxalate, and it has also been used to generate transgenic, pathogen-resistant crops. This protein has now been purified and three types of crystals grown. X-ray analysis shows that the symmetry present in these crystals is consistent with a hexameric arrangement of subunits, probably a trimer of dimers. This structure may be similar to that found in the related seed storage proteins.

【 授权许可】

Unknown