Evidence for the specific interaction of a lipid molecule with rhodopsin which is altered in the transition to the active state metarhodopsin II

FEBS Letters

Siebert, Friedrich²  Beck, Mareike²  Sakmar, Thomas P¹ 

[1] Laboratory of Molecular Biology and Biochemistry, Howard Hughes Medical Institute, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA;Institut für Biophysik und Strahlenbiologie, Albert-Ludwigs-Universität, Albertstr. 23, D-79104 Freiburg, Germany

关键词: Rhodopsin;    G protein coupled receptor;    Lipid;    Lipid-protein interaction;    FTIR;    Fourier transform infrared;    GPCR;    G protein coupled receptor;    MII;    metarhodopsin II;    PC;    phosphatidylcholine;   

DOI  :  10.1016/S0014-5793(98)01156-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Comparing the FTIR difference spectra of the rhodopsin→metarhodopsin II transition in membranes and in dodecylmaltoside detergent, characteristic variations are observed between 1715 and 1750 cm⁻¹. By repeating the measurements with the rhodopsin mutant D83N/E122Q, the spectral variation between the samples in membranes versus detergent could be assigned to a difference band at 1743(+)/1724(−) cm⁻¹, which does not exhibit a deuteration-induced downshift. We provide evidence that this band is probably caused by the C=O stretch of only one ester group of one lipid molecule. This group interacts with the dark state of rhodopsin, whereas in metarhodopsin II, the lipid molecule behaves as if it were in the bulk lipid phase.

【 授权许可】

Unknown