Characterization of a heavy metal ion transporter in the lysosomal membrane

FEBS Letters

Beerens, Cecile E.M.T¹  Mancini, Grazia M.S¹  de Gast, Izaäk L¹  Snijders, Saskia¹  Havelaar, Adrie C¹  Verheijen, Frans W¹ 

[1] Department of Clinical Genetics, Erasmus University, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands

关键词: Lysosomal transporter;    Heavy metal ion;    Copper;    Silver;    P-type ATPase;    Wilson disease;    VacA;    vacuolating cytotoxin A;    TPA;    12-O-tetradecanoylphorbol-13-O-acetate;    DMSO;    dimethyl sulfoxide;    NaBu;    sodium butyrate;    EGF;    human epidermal growth factor;   

DOI  :  10.1016/S0014-5793(98)01133-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lysosomes are thought to play a role in various aspects of heavy metal metabolism. In the present study we demonstrate for the first time the presence of a heavy metal ion transport protein in the lysosomal membrane. Uptake of radioactive silver both in highly purified lysosomal membrane vesicles and in purified intact lysosomes showed the typical kinetics of a carrier-mediated process. This transport was stimulated by ATP hydrolysis, and showed specificity for Ag⁺, Cu²⁺, and Cd²⁺. All biochemical properties of this lysosomal metal ion transporter could classify it as a heavy metal transporting P-type ATPase. Long Evans Cinnamon (LEC) rats, an animal model for the copper transport disorder Wilson disease, showed normal lysosomal silver transport.

【 授权许可】

Unknown