Preparation of basal cell membranes for scanning probe microscopy

FEBS Letters

Vinckier, A³  Murer, H⁴  Ziegler, U³  Biber, J⁴  Semenza, G⁵  Zeisel, D¹  Groscurth, P³  Kernen, P² 

[1] Department of Organic Chemistry, ETH Zurich, Zurich, Switzerland;EMPA, St. Gallen, Switzerland;Institute of Anatomy, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland;Institute of Physiology, University of Zurich, Zurich, Switzerland;Department of Biochemistry, ETH Zurich, Zurich, Switzerland

关键词: Atomic force microscopy;    Basal cell membrane;    Confocal laser scanning microscopy;    Cytoskeleton;    Scanning probe microscopy;    AFM;    atomic force microscopy;    FITC;    fluorescein-5-isothiocyanate;    MDCK;    Madine-Darby canine kidney;    PBS;    phosphate buffered saline;    SNOM;    scanning near field optical microscopy;    SPM;    scanning probe microscopy;   

DOI  :  10.1016/S0014-5793(98)01118-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Scanning probe microscopy has the potential for investigating membranes in a physiological environment. We prepared with a lysis-squirting protocol basal cell membranes, that are suitable for scanning probe microscopy. Investigations using atomic force microscopy under liquid revealed cellular filaments which correlated perfectly with fluorescently stained actin filaments. Globular structures with a diameter as little as 10 nm could be resolved by stripping cytoplasmic components from the membranes. Therefore, cytoplasmic sides of supported basal cell membranes prove useful to gain high resolution with scanning probe microscopy in studies of plasma membrane associated structures and processes under buffer solution.

【 授权许可】

Unknown