The promoter of human p22/PACAP response gene 1 (PRG1) contains functional binding sites for the p53 tumor suppressor and for NFκB

FEBS Letters

Trauzold, Anna¹  Arlt, Alexander¹  Schäfer, Heiner¹  Diebel, Julia¹  Schmidt, Wolfgang E.¹ 

[1]Laboratory of Molecular Gastroenterology and Hepatology, 1st Department of Medicine, Christian Albrechts University of Kiel, Schittenhelmstr. 12, D-24105 Kiel, Germany

关键词: Transcription factor;    Growth regulation;    Tumor cell;    Stress response;   

DOI  :  10.1016/S0014-5793(98)01109-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe functional binding sites for the tumor suppressor p53 and for NFκB residing in the promoter of the novel human early response gene p22/PRG1 (IEX-1/DIF-2). Gel shift and supershift assays demonstrate binding of p53 and NFκB to their corresponding sites in vitro. CAT-reporter gene assays show transactivation of the human p22/PRG1 promoter by p53 in Hep3B cells stably transfected with a temperature-sensitive mutant p53, but not in p53-deficient Hep3B cells. TNFα induced NFκB dependent transactivation was shown in HepG2 cells or in 818-4 pancreatic cancer cells. These data imply that human p22/PRG1 is a target gene for p53 and NFκB involved in growth regulation and stress response.

【 授权许可】

Unknown