Genome-wide analysis of Dof transcription factors and their response to cold stress in rice (Oryza sativa L.)

BMC Genomics

Hongtao Xiang¹  Jia Liu²  Chunlai Liu²  Qinglin Meng²  Baohua Su²  Ligong Ma²  Yichu Li²  Fengmei Shi²  Yu Liu² 

[1] Institute of Farming and Cultivation, Heilongjiang Academy of Agricultural Sciences, 150086, Harbin, China;Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, 150086, Harbin, China;

关键词: Cold;    Dof;    Expression profiling;    Rice;    Stress response;    Transcription factor;   

DOI  :  10.1186/s12864-021-08104-0

来源: Springer

【 摘 要 】

BackgroundRice (Oryza sativa L.) is a food crop for humans worldwide. However, temperature has an effect during the vegetative and reproductive stages. In high-latitude regions where rice is cultivated, cold stress is a major cause of yield loss and plant death. Research has identified a group of plant-specific transcription factors, DNA binding with one zinc fingers (DOFs), with a diverse range of functions, including stress signaling and stress response during plant growth. The aim of this study was to identify Dof genes in two rice subspecies, indica and japonica, and screen for Dof genes that may be involved in cold tolerance during plant growth.ResultsA total of 30 rice Dofs (OsDofs) were identified using bioinformatics and genome-wide analyses and phylogenetically analyzed. The 30 OsDOFs were classified into six subfamilies, and 24 motifs were identified based on protein sequence alignment. The chromosome locations of OsDofs were determined and nine gene duplication events were identified. A joint phylogenetic analysis was performed on DOF protein sequences obtained from four monocotyledon species to examine the evolutionary relationship of DOF proteins. Expression profiling of OsDofs from two japonica cultivars (Longdao5, which is cold-tolerant, and Longjing11, which is cold-sensitive) revealed that OsDof1 and OsDof19 are cold-inducible genes. We examined the seed setting rates in OsDof1- and OsDof19-overexpression and RNAi lines and found that OsDof1 showed a response to cold stress.ConclusionsOur investigation identified OsDof1 as a potential target for genetic breeding of rice with enhanced cold tolerance.

【 授权许可】

CC BY