Overexpression of the FGF‐2 24‐kDa isoform up‐regulates IL‐6 transcription in NIH‐3T3 cells

FEBS Letters

Arnaud, Emmanuelle¹  Delrieu, Isabelle¹  Bayard, Francis¹  Ferjoux, Géraldine¹  Faye, Jean Charles¹ 

[1] Laboratoire d'Endocrinologie et Communication Cellulaire, INSERM U397, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France

关键词: Fibroblast growth factor-2;    Interleukin-6;    NIH-3T3 cell;    Interleukin-6 promoter;    Gene regulation;   

DOI  :  10.1016/S0014-5793(98)01086-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated NIH-3T3 cell lines overexpressing the nuclear 24-kDa isoform of fibroblast growth factor (FGF)-2 and characterized its regulatory effect on the expression of interleukin-6 (IL-6) in these cells. The clone pRF5 expressing the highest level was able to grow in 1% serum medium to a high saturation density and acquired a radioresistance advantage. In pRF5 and another clone pRF1, IL-6 RNA levels were markedly increased. Studies with IL-6 promoter constructs revealed that IL-6 gene up-regulation occurred at the transcriptional level and did not involve the AP-1 binding site. Exogenously added 18-kDa isoform of FGF-2 (100 ng/ml) produced down-regulation of IL-6 involving an AP-1 binding site, thus suggesting a receptor-independent pathway for the intracellular 24-kDa isoform.

【 授权许可】

Unknown