FEBS Letters | |
Overexpression of the FGF‐2 24‐kDa isoform up‐regulates IL‐6 transcription in NIH‐3T3 cells | |
Arnaud, Emmanuelle1  Delrieu, Isabelle1  Bayard, Francis1  Ferjoux, Géraldine1  Faye, Jean Charles1  | |
[1] Laboratoire d'Endocrinologie et Communication Cellulaire, INSERM U397, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France | |
关键词: Fibroblast growth factor-2; Interleukin-6; NIH-3T3 cell; Interleukin-6 promoter; Gene regulation; | |
DOI : 10.1016/S0014-5793(98)01086-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have isolated NIH-3T3 cell lines overexpressing the nuclear 24-kDa isoform of fibroblast growth factor (FGF)-2 and characterized its regulatory effect on the expression of interleukin-6 (IL-6) in these cells. The clone pRF5 expressing the highest level was able to grow in 1% serum medium to a high saturation density and acquired a radioresistance advantage. In pRF5 and another clone pRF1, IL-6 RNA levels were markedly increased. Studies with IL-6 promoter constructs revealed that IL-6 gene up-regulation occurred at the transcriptional level and did not involve the AP-1 binding site. Exogenously added 18-kDa isoform of FGF-2 (100 ng/ml) produced down-regulation of IL-6 involving an AP-1 binding site, thus suggesting a receptor-independent pathway for the intracellular 24-kDa isoform.
【 授权许可】
Unknown
【 预 览 】
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RO201912020306562ZK.pdf | 241KB | download |