Cloning, expression, and localization of a novel γ‐adaptin‐like molecule

FEBS Letters

Sheff, David¹  Mellman, Ira¹  Bonifacino, Juan S.²  Yamamoto, Ellen¹  Lewin, David A.¹  Ooi, Chean Eng²  Whitney, J.Andrew¹  Webster, Paul¹  Chicione, Linda M.¹ 

[1] Yale University School of Medicine, Department of Cell Biology, P.O. Box 208002, New Haven, CT 06520-8002, USA;Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD 20892, USA

关键词: Adaptin;    Adaptor;    Golgi;    Human;    Mouse;   

DOI  :  10.1016/S0014-5793(98)01083-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe the cloning, expression, and localization of γ₂-adaptin, a novel isoform of γ-adaptin. The predicted human and mouse γ₂-adaptin proteins are ∼90 kDa and 64.4% and 61.7% identical to γ-adaptin, respectively. γ₂-Adaptin was localized to the Golgi, its localization distinct from γ-adaptin. The membrane association of γ- and γ₂-adaptin could further be distinguished by differential sensitivity to the fungal metabolite brefeldin A, γ₂-adaptin binding being insensitive to drug treatment. Together, these results suggest that γ₂-adaptin plays a role in membrane transport distinct from that played by γ-adaptin.

【 授权许可】

Unknown