FEBS Letters | |
Cloning, expression, and localization of a novel γ‐adaptin‐like molecule | |
Sheff, David1  Mellman, Ira1  Bonifacino, Juan S.2  Yamamoto, Ellen1  Lewin, David A.1  Ooi, Chean Eng2  Whitney, J.Andrew1  Webster, Paul1  Chicione, Linda M.1  | |
[1] Yale University School of Medicine, Department of Cell Biology, P.O. Box 208002, New Haven, CT 06520-8002, USA;Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD 20892, USA | |
关键词: Adaptin; Adaptor; Golgi; Human; Mouse; | |
DOI : 10.1016/S0014-5793(98)01083-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We describe the cloning, expression, and localization of γ2-adaptin, a novel isoform of γ-adaptin. The predicted human and mouse γ2-adaptin proteins are ∼90 kDa and 64.4% and 61.7% identical to γ-adaptin, respectively. γ2-Adaptin was localized to the Golgi, its localization distinct from γ-adaptin. The membrane association of γ- and γ2-adaptin could further be distinguished by differential sensitivity to the fungal metabolite brefeldin A, γ2-adaptin binding being insensitive to drug treatment. Together, these results suggest that γ2-adaptin plays a role in membrane transport distinct from that played by γ-adaptin.
【 授权许可】
Unknown
【 预 览 】
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RO201912020306556ZK.pdf | 1775KB | download |