Effect of CRF and related peptides on calcium signaling in human and rodent melanoma cells

FEBS Letters

Choudhry, Mashkoor A³  Fazal, Nadeem¹  Sayeed, Mohammed M³  Slominski, Andrzej¹  Wei, Edward T² 

[1] Department of Pathology, Medical Center, Loyola University, 2160 First South Avenue, Maywood, IL 60153, USA;School of Public Health, University of California, Berkeley, CA, USA;Department of Physiology, Medical Center, Loyola University, 2160 First South Avenue, Maywood, IL 60153, USA

关键词: Corticotropin releasing factor;    Urocortin;    Sauvagine;    Receptor;    Calcium;    Malignant melanocyte;   

DOI  :  10.1016/S0014-5793(98)01067-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Corticotropin releasing factor (CRF) induces a rapid, within seconds, and dose-dependent increase in the intracellular Ca²⁺ in both human and hamster melanoma cells. This effect is inhibited by depletion of extracellular calcium using 3 mM EGTA and is attenuated by the CRF receptor antagonist, α-helical-CRF(9-41). Other peptides of the CRF superfamily, sauvagine and urocortin, also induce increases in cytoplasmic calcium concentration but at higher concentrations than CRF. We conclude that malignant melanocytes express CRF receptors, which are coupled to activation of plasma membrane calcium channels.

【 授权许可】

Unknown