FEBS Letters | |
Novel defensin subfamily from spinach (Spinacia oleracea) | |
Garcı́a-Olmedo, Francisco1  Segura, Ana1  Molina, Antonio1  Moreno, Manuel1  | |
[1] Laboratorio de Bioquı́mica y Biologı́a Molecular, Departamento de Biotecnologı́a-UPM, ETS Ingenieros Agrónomos, E-28040 Madrid, Spain | |
关键词: Antimicrobial peptide; Innate immunity; Plant defense; Plant defensin; Plant pathogen; Spinach; | |
DOI : 10.1016/S0014-5793(98)01060-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Antimicrobial peptides (So-D1-7) were isolated from a crude cell wall preparation from spinach leaves (Spinacia oleracea cv. Matador) and, judged from their amino acid sequences, six of them (So-D2-7) represented a novel structural subfamily of plant defensins (group IV). Group-IV defensins were also functionally distinct from those of groups I–III. They were active at concentrations <20 μM against Gram-positive (Clavibacter michiganensis) and Gram-negative (Ralstonia solanacearum) bacterial pathogens, as well as against fungi, such as Fusarium culmorum, F. solani, Bipolaris maydis, and Colletotrichum lagenarium. Fungal inhibition occurred without hyphal branching. Group-IV defensins were preferentially distributed in the epidermal cell layer of leaves and in the subepidermal region of stems.
【 授权许可】
Unknown
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