Novel defensin subfamily from spinach (Spinacia oleracea)

FEBS Letters

Garcı́a-Olmedo, Francisco¹  Segura, Ana¹  Molina, Antonio¹  Moreno, Manuel¹ 

[1] Laboratorio de Bioquı́mica y Biologı́a Molecular, Departamento de Biotecnologı́a-UPM, ETS Ingenieros Agrónomos, E-28040 Madrid, Spain

关键词: Antimicrobial peptide;    Innate immunity;    Plant defense;    Plant defensin;    Plant pathogen;    Spinach;   

DOI  :  10.1016/S0014-5793(98)01060-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antimicrobial peptides (So-D1-7) were isolated from a crude cell wall preparation from spinach leaves (Spinacia oleracea cv. Matador) and, judged from their amino acid sequences, six of them (So-D2-7) represented a novel structural subfamily of plant defensins (group IV). Group-IV defensins were also functionally distinct from those of groups I–III. They were active at concentrations <20 μM against Gram-positive (Clavibacter michiganensis) and Gram-negative (Ralstonia solanacearum) bacterial pathogens, as well as against fungi, such as Fusarium culmorum, F. solani, Bipolaris maydis, and Colletotrichum lagenarium. Fungal inhibition occurred without hyphal branching. Group-IV defensins were preferentially distributed in the epidermal cell layer of leaves and in the subepidermal region of stems.

【 授权许可】

Unknown