Glucocorticoids decrease cytochrome c oxidase activity of isolated rat kidney mitochondria

FEBS Letters

Jolliet, Pascale¹  Tillement, Jean-Paul¹  Morin, Christophe¹  Simon, Nicolas¹  Urien, Saı̈k¹  Zini, Roland¹ 

[1] Départment de Pharmacologie, Faculté de Médecine de Paris XII, 8 rue du Général Sarrail, F-94010 Créteil, France

关键词: Mitochondria;    Glucocorticoid;    Oxidative phosphorylation;    Cytochrome c oxidase;   

DOI  :  10.1016/S0014-5793(98)01033-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The importance of mitochondria is rising as a target in pathologic processes such as ischemia. We have investigated the effects of hydrocortisone, prednisolone, dexamethasone and triamcinolone on oxidative phosphorylation, Ca²⁺ fluxes, swelling and membrane potentials in isolated kidney mitochondria. The measurement of respiration state 3 showed a significant decrease in presence of glucocorticoids whereas the other respiration states were not modified. When mitochondria were uncoupled and either the complexes III and IV or the complex IV were stimulated, the O₂ consumption was decreased by glucocorticoids. These results suggest the cytochrome c oxidase is a target of the glucocorticoid effect on the respiratory chain. Indeed, the other mitochondrial functions investigated were unchanged, ruling out a direct effect on Ca²⁺ fluxes or swelling. A regulation of cytochrome c oxidase activity by glucocorticoids will be of particular interest in pathology involving metabolic insult.

【 授权许可】

Unknown