FEBS Letters | |
The peptide HDEF as a new retention signal is necessary and sufficient to direct proteins to the endoplasmic reticulum | |
Kaletta, Karin2  Kunze, Gotthard1  Kunze, Irene1  Köck, Margret2  | |
[1] Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, Germany;Martin-Luther-Universität Halle-Wittenberg, Biozentrum, Weinbergweg 22, D-06120 Halle, Germany | |
关键词: Chitinase; Endoplasmic reticulum and retention; Heterologous expression; Ribonuclease; Saccharomyces cerevisiae; Tomato; | |
DOI : 10.1016/S0014-5793(98)01013-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The key feature of tomato RNase LX localised solely outside the vacuole is the C-terminal peptide HDEF which is very similar to known endoplasmic reticulum (ER) retention signals. For functional testing of the ER-targeting ability of HDEF, different constructs including the complete RNase LX, two truncated forms without HDEF and the truncated chitinase FB7-1ΔVTP C-terminally flanked by HDEF, were expressed in Saccharomyces cerevisiae. The majority of RNase and chitinase, both containing HDEF, accumulates within the ER. However, the truncated constructs without the peptide are released into the medium. We provide compelling evidence that peptide HDEF at the C-terminus of secretory plant proteins is a new ER retention signal in yeast and most likely in plants.
【 授权许可】
Unknown
【 预 览 】
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RO201912020306489ZK.pdf | 205KB | download |