Oxidative decomposition of malonic acid as basis for the action of manganese peroxidase in the absence of hydrogen peroxide

FEBS Letters

Jäger, M.G.¹  Fritsche, W.³  Ziegenhagen, D.³  Hatakka, A.²  Vares, T.²  Hofrichter, M.³  Friedrich, M.¹ 

[1] Friedrich Schiller University of Jena, Institute of Inorganic and Analaytic Chemistry, August-Bebel-Straße 2, D-07743 Jena, Germany;Department of Applied Chemistry and Microbiology, P.O. Box 56, Biocenter 1 (Viikinkaari 9), University of Helsinki, FIN-00014 Helsinki, Finland;Friedrich Schiller University of Jena, Institute of Microbiology, Department of Technical Microbiology, Philosophenweg 12, D-07743 Jena, Germany

关键词: Manganese peroxidase;    Malonic acid;    Peroxyl radical;    Hydroperoxide;    Phlebia radiata;    Nematoloma frowardii;   

DOI  :  10.1016/S0014-5793(98)01023-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Manganese peroxidase (MnP) from the ligninolytic basidiomycetes Phlebia radiata and Nematoloma frowardii was found to decompose malonate oxidatively in the absence of H₂O₂ in a reaction system consisting of the enzyme, sodium malonate and MnCl₂. The enzymatic oxidation resulted in a substantial decrease in malonate concentration and the formation of CO₂, oxalate, glyoxylate and formate. Simultaneously with the decomposition of malonate, Mn(II) was oxidized to Mn(III) leading to high transient concentrations of the latter. MnP action in the absence of H₂O₂ started slowly after a lag period of 3 h. The lag period was considerably shortened after a single addition of Mn(III). Superoxide dismutase and catalase inhibited the enzymatic reaction partly, ascorbate completely. ESR studies demonstrated the formation of a carbon-centered radical during the course of the reaction. We propose that the latter generates peroxides that can be used by MnP to oxidize Mn(II) to Mn(III).

【 授权许可】

Unknown