Soluble IL‐1 receptor type I binds to human dermal fibroblasts and induces calcium flux

FEBS Letters

Ochsenbein, Regula M.⁴  Loetscher, Marcel¹  Lottaz, Daniel³  Waelti, Ernst R.²  Wiesmann, Ueli N.⁵  Spörri, Bernhard⁶  Bickel, Matthias⁶ 

[1] Theodor Kocher Institute, University of Bern, Bern, Switzerland;Department of Immunopathology, University of Bern, Bern, Switzerland;Institute of Molecular Biology and Biochemistry, University of Bern, Bern, Switzerland;Department of Pharmacology, University of Bern, Bern, Switzerland;Department of Pediatrics, University of Bern, Bern, Switzerland;Laboratory of Oral Cell Biology, Freiburgstrasse 7, CH-3010 Bern, Switzerland

关键词: Interleukin-1;    Soluble receptor;    Calcium;    Signal transduction;    Fibroblast;    IL-1;    interleukin-1;    IL-1ra;    interleukin-1 receptor antagonist;    IL-1R I;    type I IL-1 receptor;    IL-1R-AcP;    IL-1R accessory protein;    IL-1sR I;    soluble IL-1 receptor type I;    BK;    bradykinin;    IL-6Rα;    soluble IL-6 receptor α chain;   

DOI  :  10.1016/S0014-5793(98)00995-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Soluble cytokine receptors appear to modify ligand concentrations by stabilizing ligands or by specifically inhibiting interactions of ligands with their membrane-bound receptors. Here we describe a new function of the soluble interleukin-1 receptor type I (IL-1sR I). This receptor induced a transient rise of intracellular free calcium concentration in human dermal fibroblasts in a dose-dependent fashion. Mobilization of calcium by IL-1sR I was abolished in the presence of an equimolar concentration of IL-1 receptor antagonist (IL-1ra). Neutralizing antibodies against IL-1β also abolished calcium mobilization stimulated with IL-1sR I indicating that IL-1β is involved. IL-1sR I bound with high affinity (K _d 1–2 nM) to the fibroblasts. In addition, IL-1sR I enhanced expression of IL-6 and IL-8 mRNA. The observation that IL-1sR I can act as a ligand and agonist for membrane IL-1 extends the concept of the ligand-receptor functions of both IL-1 and IL-1sR I and adds a new dimension to the cytokine network.

【 授权许可】

Unknown