Biosynthesis of d‐aspartate in mammalian cells

FEBS Letters

Homma, Hiroshi¹  Lee, Jen-Ai¹  Yamada, Ryo-hei²  Iwatsubo, Takeshi¹  Imai, Kazuhiro¹  Long, Zhiqun¹  Santa, Tomofumi¹  Fukushima, Takeshi¹ 

[1] Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan;Department of BioEngineering, Nagaoka University of Technology, Nagaoka, Niigata 940-21, Japan

关键词: d-Amino acid;    d-Aspartate;    Biosynthesis;    d-Serine;    PC12;   

DOI  :  10.1016/S0014-5793(98)00986-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this communication, we demonstrate that d-aspartate (d-Asp) is synthesized in pheochromocytoma cells (PC12). To our knowledge this is the first report of biosynthesis of d-Asp in mammalian cells. Synthesis of d-Asp was demonstrated by its time-dependent accumulation in the cell culture, and by the fact that this accumulation was proportional to the number of inoculated cells. d-Asp in PC12 cells was identified by (i) co-elution with authentic d-Asp on two different HPLC columns, an octadesyl silica column and a Pirkle-type chiral column, (ii) reversed elution order of d-Asp and l-Asp on another Pirkle-type chiral column with an opposite configuration, and (iii) sensitivity to d-Asp oxidase. In the cells the amount of d-Asp was approx. 12–14% of total Asp and no other investigated d-amino acid was detected. The amount of d-Asp did not increase during the culture of mouse 3T3 fibroblasts and human neuroblastoma NB-1 cells. Immunocytochemical staining with anti-d-Asp antiserum demonstrated that d-Asp synthesized is present in the cytoplasm of the cells.

【 授权许可】

Unknown